Abstracts (first author)

Poster 

Next Generation Sequencing reveals high complexity of the Major Histocompability Complex class I exon 3 in sedge warbler

Author(s): Biedrzycka A, Radwan J, Westerdahl H

Summary:

The major Histocompability Complex (MHC) class I genes have received a considerable of interest from evolutionary biologists. Their role in parasite recognition and extreme polymorphism makes these genes a paradigm for studying genetic effects on survival, mate choice and pathogen resistance. Exon 3 of MHC class I genes encodes a part of the domain that binds and presents peptides from pathogens. Species possessing many highly polymporphic MHC loci are potentially under strong natural selection from parasites. Determining selection acting on MHC in natural population of such species over extended periods of time offers a great opportunity to reveal mechanisms maintaining MHC diversity. Here we present initial characterization of MHC class I genes diversity in a sedge warbler Acrocephalus schoenobaenus from population inhabiting natural wetlands in the Nida valley, SE Poland. We designed primers and determined diversity at MHC class I exon 3 both in cDNA and gDNA of 4 unrelated individuals with use of Next Generation Sequencing methods. We found extreme diversity at the MHC class I exon 3 of the sedge warbler. This data will be used for designing specific primers amplifying functional alleles of MHC class I exon 3 in sedge warbler. Subsequently, we are going to infer long-term selection on MHC class I in the species.



Contacts

Chairman: Octávio S. Paulo
Tel: 00 351 217500614 direct
Tel: 00 351 217500000 ext22359
Fax: 00 351 217500028
email: mail@eseb2013.com

Address

XIV Congress of the European Society for Evolutionary Biology

Organization Team
Department of Animal Biology (DBA)
Faculty of Sciences of the University of Lisbon
P-1749-016 Lisbon
Portugal

Website

Computational Biology & Population Genomics Group 
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