Abstracts (first author)
Meiotic drive in the yellow fever mosquito Aedes aegyptiPDF
Selfish genetic elements enhance their own transmission to the organism’s progeny in a disproportionate fashion. Sex chromosome meiotic drive normally involves X chromosomes and results in female biased sex ratios, but in the yellow mosquito Aedes aegypti a meiotic drive gene is tightly linked to the male chromosome and distorts the sex ratio towards males. This distortion can only be seen when males bearing the meiotic drive gene (D) are crossed to females from other populations. That is because a dominant insensitive gene (I) has evolved in females of the populations where D is present, preventing the sex ratio distortion while females from other geographical regions are commonly sensitive (s) to D. We crossed females of a line that is highly sensitive to meiotic drive to a line carrying the drive gene. Using restriction-site associated DNA (RAD) tags followed by deep sequencing, we were able to both map the meiotic drive gene and genes elsewhere in the genome which suppress meiotic drive. We then backcrossed the meiotic drive gene into a genetic background without any suppressors and repeated the experiment. This allowed us to map the meiotic drive gene with greater precision.