Abstracts (first author)

Poster 

Comparative genomics of insect endosymbionts extracted from single hosts

Author(s): Tamarit D, Klasson L, Andersson SGE

Summary:

Insect endosymbionts represent the paradigm of bacterial adaptation to a host-dependent life and their study contributes to our understanding on microbial ecology and genome evolution. Their informative nature is nonetheless counterpoised to their inaccessibility. Here we demonstrate that single-host endosymbiotic communities can be studied from next-generation sequencing data after purification and whole-genome amplification of endosymbionts. We focus on the comparative genomic study of two samples belonging to pure Hamiltonella defensa sequences extracted from two individual aphids from the tribe Macrosiphini. The assembly of their genomes show that they are 1.8 and 2.0 Mb-long, respectively, and their gene content is shown to be similar to that of other H. defensa genomes. We used this new data to fully resolve the phylogenetic relationships between the known H. defensa and related enterobacteria, and to reconstruct the history of gene gains and losses in their evolutionary lineage. Finally, we performed an in-depth analysis of the genome content and evolution of the APSE bacteriophage, inserted in the H. defensa genomes, which has a remarkable ecological importance in protecting the aphid host against parasitoid wasp infections.



Contacts

Chairman: Octávio S. Paulo
Tel: 00 351 217500614 direct
Tel: 00 351 217500000 ext22359
Fax: 00 351 217500028
email: mail@eseb2013.com

Address

XIV Congress of the European Society for Evolutionary Biology

Organization Team
Department of Animal Biology (DBA)
Faculty of Sciences of the University of Lisbon
P-1749-016 Lisbon
Portugal

Website

Computational Biology & Population Genomics Group 
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