Emily M. Lemmon
Assistant Professor in Department of Biological Science at Florida State UniversityFlorida State University
Department of Biological Science
Ecology and Evolution
319 Stadium Drive, P.O. Box 3064295
Tallahassee, FL, 32306-4295
United States
Website
Abstracts (first author)
Anchored Phylogenomics: Accelerating the Resolution of Life
Summary:
The field of phylogenomics is undergoing a revolution, enabled by new methods of data collection that leverage both genomic resources and recent advances in DNA sequencing. We developed a cost-efficient and rapid approach to obtaining data from 100s to 1000s of loci and individuals for deep and shallow phylogenetic studies. Specifically, we designed probes for target hybrid enrichment of 100s of loci in conserved anchor regions of genomes (flanked by less conserved regions). We enriched genomic DNA libraries for these anchor regions, and sequenced these targets using high-throughput sequencing. The resulting data sets contain 100s of loci with low levels of missing data and high levels of phylogenetic information across taxonomic scales and produce phylogenies with high levels of resolution. This approach is expediting resolution of deep-scale portions of the Tree of Life and greatly accelerating resolution of the large number of shallow clades that remain unresolved. The combination of low cost (∼1% of the cost of traditional Sanger sequencing and ∼3.5% of the cost of high-throughput amplicon sequencing for projects on the scale of 500 loci × 100 individuals) and rapid data collection (∼2 weeks of laboratory time) make this approach tractable even for researchers working on systems with limited genomic resources. Here, I present new work from vertebrates and unpublished data from various non-vertebrate (e.g., Coleoptera, Hymenoptera, etc.) and plant (e.g., Angiosperm) clades; I also discuss future directions and new applications.