Abstracts (first author)
Pisum genome variability detected by AFLP and RGA profiling
Pea (P. sativum L.) is an important legume grown and consumed extensively worldwide. In the present study for the first time two different multiloci techniques - AFLP and RGA-profiling have been used for genetic diversity assessment and taxa boundaries delimitation in Pisum species. RGA-profiling technique was used to determine NBS-LRR plant pathogen resistance genes polymorphism. In contrast, AFLP technique allows to investigate mostly evolutionary neutral genome sequences. Both approaches can provide comprehensive genome diversity information. Genetic diversity of 110 Pisum accessions of different origin representing P. fulvum and P. sativum species, including abyssinicum, asiaticum, transcaucasicum and arvense accessions with unclear taxonomic status was analyzed using AFLP and RGA- profiling. By using preselected primer combinations 405 AFLP and 171 RGA polymorphic bands have been detected. As a result, for each pea genotype unique AFLP and RGA-specific patterns have been revealed. The dendrogram and PCA based on calculated GD values revealed two distinct groups corresponding to P. sativum and P. fulvum. Other taxa formed one mixed group with P. sativum accessions. Notably, on AFLP, PCA-plot all P. sativum formed continuum with two main poles, largely represented Asian or European accessions. On RGA-profiling PCA-plot, no geographical and taxonomical differentiations within P.sativum accessions were determined. No correlation between pea accession grouping and resistance status or ability to interact with arbuscular mycorrhiza was found. Obtained data suggested that genus Pisum is composed only of two clear species: P. sativum and P. fulvum. There is no differentiation between abyssinicum, asiaticum, arvense, transcaucasicum and sativum,that confirm their subspecies taxonomic status. Molecular characterization of Pisum genetic diversity levels may help to identify potential new sources useful in pea breeding. The work was supported by FRP "Biological Diversity".