Abstracts (first author)
Genetic diversity of a relic moose population, Alces alces in the Biebrza Valley, NE PolandPDF
Moose (Alces alces) are highly mobile mammals that occur across arboreal regions of North America, Europe and Asia. Hundertmark et al. (2002) identified three distinct mtDNA lineages of moose in these regions. The distribution of these lineages indicated a geographic structure associated with a glacial history. To examine the genetic diversity and population structure of moose in northeastern Poland we analyzed mtDNA sequences of 1747 bp long (control region combined with cytochrome b) of 500 individuals from 13 populations. Our study revealed presence of 12 haplotype belonging to the European lineage, from which only five haplotypes were early detected by Hundertmark et. al. (2002) exclusively among moose from Scandinavian Peninsula. The haplotype (h) and nucleotide (π) diversity values for analyzed moose samples were respectively 0,76 and 1,2%. Altogether, 27 sites were variable and all substitutions appeared to be transitions. In our study the haplotype H1 was mainly present in the Biebrza Valley and it was divergented from the remaining European haplotypes. Phylogenetic relationships were computed using a Neighbor-Joining (NJ) and a Maximum Likelihood (ML) trees. In both analysis the clade contained the unique haplotype H1 from the Biebrza Valley was the most distinct within the European lineage. Net sequence divergence between these clade and the others European haplotype amount 1,4%, which means moose from the Biebrza Valley separated 23000 years ago, eg during the Last Glacial Maximum (LGM). We also found that genetic differentiation between several moose populations in northeastern Poland was great, for example the ФST value between two neighbouring populations: from the Biebrza Valley and the Augustowska Forest was 0.55. In addition, we analyzed others classes of molecular markers: 11 microsatellite loci and markers located on Y chromosome (SRY and YCATs) which also confirmed relic character of moose from the Biebrza Valley.