Abstracts (first author)

Talk 

Recombination landscape of Ficedula flycatchers

Author(s): Kawakami T, Backström N, Olason PI, Burri R, Husby A, Smeds L, Qvarnström A, Ellegren H

Summary:

Characterizing variation in recombination rates between and within species is essential for understanding the patterns of gene flow, the efficacy of selection, and the variation in genetic divergence and diversity. The Natural pedigree of collared flycatchers (Ficedula albicollis) available from the Baltic island of Öland provides an exclusive opportunity to investigate variation in the rate of recombination in wild bird populations. Using a newly developed custom 50,000 SNP chip, we constructed a high-density genetic linkage map of the collared flycatcher. Our linkage analysis placed 4,951 SNPs in 33 linkage groups, corresponding to 29 autosomes and the Z chromosome. The total genetic distance was 3,096 cM with higher recombination rate in males than females (3,226 cM in males, 2,970 cM in females). Recombination rates substantially vary between chromosomes (mean recombination rate per chromosome = 2.04 ~ 6.97 cM/Mb) as well as within chromosomes and are generally higher toward the end of chromosomes. In addition to the collared flycatcher pedigree samples, we have genotyped multiple populations of collared flycatchers across Europe to characterize the pattern of linkage disequilibrium (LD) and estimate population-scaled recombination rate (rho). Population-specific recombination patterns and association with underlying molecular features will be discussed.


Video


Abstracts (coauthor)

The landscape(s) of genome divergence in Ficedula flycatchers

Author(s): Burri, R, Smeds L, Olason PI, Backstrom N, Kawakami T, Kunstner A, Makinen H, Nadachowska-Brzyska K, Qvarnström A, Uebbing S, Wolf JBW, Saetre G, Saether SA, Hogner S, Bures S, Garamszegi LZ, Moreno J, Ruzic M, Torok J, Ellegren H

Summary:

Unravelling the landscapes of genome divergence between individuals, populations, and species is key to understand the genomic architecture of biological diversification, and the forces driving its evolution. In the present study we make use of the recently available flycatcher genome (Ellegren et al. 2012, Nature), to study the genomic divergence between the naturally hybridizing but reproductively isolated collared flycatcher (Ficedula albicollis) and pied flycatcher (F. hypoleuca). Population genomic analysis of each 10 genomes of both species reveal a highly heterogeneous landscape of genome divergence with about 50 ‘divergence islands’ that show ~50-fold higher differentiation than the genomic background. The non-randomly distributed divergence islands are characterized by reduced nucleotide diversity, skewed allele-frequency spectra, elevated linkage disequilibrium and reduced proportions of shared polymorphisms in both species, indicating parallel episodes of selection. The high incidence of divergence islands with genomic regions resistant to sequence assembly, potentially including centromeres and telomeres, indicate that complex repeat structures may drive species divergence. In order to identify the molecular and evolutionary forces driving the evolution of divergence islands in Ficedula flycatchers, these insights are complemented with recent results from population genomic analyses of an additional 200 flycatcher genomes from multiple populations and species.

Contacts

Chairman: Octávio S. Paulo
Tel: 00 351 217500614 direct
Tel: 00 351 217500000 ext22359
Fax: 00 351 217500028
email: mail@eseb2013.com

Address

XIV Congress of the European Society for Evolutionary Biology

Organization Team
Department of Animal Biology (DBA)
Faculty of Sciences of the University of Lisbon
P-1749-016 Lisbon
Portugal

Website

Computational Biology & Population Genomics Group 
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